A novel magnetic nitrogen-doped reduced graphene oxide (Fe < sub > 3 < /sub > O < sub > 4 < /sub > @ N-RGO) had been fabricated for the first time on the basis of a simple solvothermal method and then was successfully applied to extract four bisphenol endocrine disruptors (Bisphenol A, bisphenol B, bisphenol F and bisphenol ) in carbonated beverages coupled with high performance liquid chromatography (HPLC). The as-prepared Fe < sub > 3 < /sub > O < sub > 4 < /sub > @ N-RGO was characterized by transmission electron microscopy (TEM), Brunner-Emmet-Teller (BET), X-ray diffraction (XRD), X-ray photoelectron spectrometer (XPS) and vibrating sample magnetometer (VSM). The introduction of nitrogen atoms not only made the wrinkle level of N-RGO increased, but also retarded the irreversible aggregation of graphene sheets. Compared with Fe < sub > 3 < /sub > O < sub > 4 < /sub > @ RGO, Fe < sub > 3 < /sub > O < sub > 4 < /sub > @ N-RGO owned larger specific surface area and more adsorption sites. Hence, Fe < sub > 3 < /sub > O < sub > 4 < /sub > @ N-RGO showed excellent extraction efficiency toward bisphenol endocrine disruptors. The analytical parameters influencing the extraction efficiency were optimized in detail. Under the optimal conditions, a satisfactory performance was obtained. The calibration lines were linear over the concentration in the range of 0.4-1000a-I1/4ga-L < sup > -1 < /sup > with determination coefficients (r < sup > 2 < /sup >) between 0.9976 and 0.9996. The limits of detection (LOD) ranged from 0.1a-I1/4ga-L < sup > -1 < /sup > to 0.2a-I1/4ga-L < sup > -1 < /sup >. The recoveries varied from 86.52 % to 101.47 % with relative standard deviations (RSDs) less than 8.59 %. Overall, the proposed method was an efi ! cient pretreatment and enrichment procedure and could be successfully applied for selective extraction and determination of bisphenol endocrine disruptors in complex matrices.
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This project is supported by the Canadian Institutes of Health Research (award #111062), Alberta Innovates - Health Solutions, and by The Metabolomics Innovation Centre (TMIC), a nationally-funded research and core facility that supports a wide range of cutting-edge metabolomic studies. TMIC is funded by Genome Alberta, Genome British Columbia, and Genome Canada, a not-for-profit organization that is leading Canada's national genomics strategy with $900 million in funding from the federal government.