Dispersive liquid-liquid microextraction (DLLME) coupled with was successfully developed for simultaneous determination of four types of phenolic environmental estrogens (PEEs), namely hexestrol (HS), Bisphenol A ( ), diethylstilbestrol (DES) and dienestrol (DS). Several parameters affecting DLLME and conditions were systematically investigated including the type and volume of extraction solvent and dispersive solvent, extraction time, salt, pH value, surfactant, buffer solution and so on. Under the optimal conditions, DLLME-CE exhibited strong enrichment ability, presenting high enrichment factors of 467, 241, 367 and 362 for HS, , DES and DS, respectively, as well as low detection limits of 0.3, 0.6, 0.6 and 0.3 I1/4g/L, respectively. Excellent linearity was achieved in the range of 2.0-150 I1/4g/L for HS and DS, and 4.0-300 I1/4g/L for and DES, with correlation coefficients R & gt; 0.9983. Recoveries ranging from 70.4 to 108.1 % were obtained with tap water, lake water and seawater samples spiked at three concentration levels and the relative standard deviations (RSDs, for n = ) were 2.1-9.7 %. This DLLME-CE method with high selectivity and sensitivity, high stability, simplicity, cost-effectiveness, eco-friendliness was proved potentially applicable for the rapid and simultaneous determination of PEEs in complicated water samples.
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This project is supported by the Canadian Institutes of Health Research (award #111062), Alberta Innovates - Health Solutions, and by The Metabolomics Innovation Centre (TMIC), a nationally-funded research and core facility that supports a wide range of cutting-edge metabolomic studies. TMIC is funded by Genome Alberta, Genome British Columbia, and Genome Canada, a not-for-profit organization that is leading Canada's national genomics strategy with $900 million in funding from the federal government.